ScienceDirect Publication: Cytokine
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BAPTA-AM, an intracellular calcium chelator, inhibits RANKL-induced bone marr...
27 Jul 2010 at 1:08pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 27 July 2010
Sigui, Zhou , Xi, Yuan , Qinghua, Liu , Xiaoqi, Zhang , Xuediao, Pan , ...
To examine the roles of intracellular calcium in RANKL-induced bone marrow macrophages (BMMs) differentiation, the effects of intracellular calcium chelator BAPTA-AM on RANKL-induced BMMs differentiation, and the activation of its relating signal proteins (MAPKs, and the PI3K/Akt) were studied. BMMs were cultured with various concentrations of BAPTA-AM in the presence of M-CSF (25ng/ml) and RANKL (25ng/ml) for 7days, osteoclastogenic ability, cytosolic free Ca2+ concentration, osteoclast survival and the expression of phosphorylated ERK1/2, SAPK/JNK, Akt and p38 MAPK were measured by TRAP staining, spectrofluorometer and Western blotting. BAPTA-AM inhibited osteoclastogenesis and osteoclast survival of BMMs by RANKL induction. In osteoclasts without...
Urinary parameters predictive of cisplatin-induced acute renal injury in dogs
24 Jul 2010 at 1:09pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 23 July 2010
J. Eric, McDuffie , Marciano, Sablad , JingYing, Ma , Sandra, Snook
A 28-day study was conducted to evaluate changes in urinary cytokine/chemokine expression levels in dogs with renal injury due to administration of cisplatin. Animals (n=17) were administered cisplatin at 0.75mg/kg/day (i.v.) for five consecutive days. Urine/serum were collected at pre-dosing, 4h post-dosing and on days 2, 3, 4, 8, 10, 14, 16, 18, 21, 23, 25, 28 and unscheduled terminations. Animals were euthanized when serum creatinine (sCr) levels measured at ?1.9mg/dL, indicating significant loss of renal function (decreased glomerular filtration rate). Relevant clinical observations included lethargy and dehydration. Pre-study sCr levels ranged from 0.6 to 0.8mg/dL; on days 1 through...
MCP-1 and MIP-1? expression in a model resembling early immune response to de...
23 Jul 2010 at 1:07pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 22 July 2010
Beatriz, Sierra , Ana B., Perez , Katrin, Vogt , Gissell, Garcia , Kathrin, Schmolke , ...
Dengue virus has become endemic in most tropical urban areas throughout the world, and DHF has appeared concomitantly with this expansion. The intensity of dengue virus replication during the early stages of infection could determine clinical outcomes; therefore, it is important to understand the impact of dengue virus infection on the earliest immune defense against microbial infection, which also strongly regulates the adaptive immune responses. This study was aimed at evaluating the expression of the CC-chemokines MIP-1?/CCL3 and MCP-1/CCL2 in peripheral blood leukocytes using an ex vivo model resembling dengue infection in vivo, in subjects with a well characterized dengue...
Interleukin-12 as an adjuvant for induction of protective antibody responses
23 Jul 2010 at 1:07pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 22 July 2010
Dennis W., Metzger
Interleukin (IL)-12 is a pivotal cytokine that strongly stimulates Th1-associated cellular immunity. It is now recognized that IL-12 also activates humoral immunity to both T-dependent and T-independent antigens. This has let to considerable interest in exploiting IL-12 as a vaccine adjuvant for protection against various bacterial and viral pathogens, particularly in the lung. Studies examining the efficacy of IL-12-mediated effects on protective antibody response in the mouse model are summarized in this review.
Cloning and characterization of rhesus IL-18 binding protein, a natural antag...
20 Jul 2010 at 1:06pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 20 July 2010
Srikanth, Yellayi , Steve, Braun , Heber G., Domingues , Nadya, Kityatana , Ramachandran, Murali , ...
IL-18 is a proinflammatory cytokine that is important for host defense, but is also involved in the pathogenesis of a number of disease processes, ranging from autoimmune disorders to atherosclerosis. IL-18 binding protein (IL-18BP) is a constitutively expressed glycoprotein that specifically neutralizes the effects of IL-18, resulting in decreased production of IFN-? and reduction in Th1 immune responses. In this study we cloned and sequenced a full-length cDNA of the rhesus IL-18BP (RhIL-18BP) from the spleen of rhesus macaques (Macaca mulatta) and compared its nucleotide and amino acid sequences to the functional murine and human IL-18BP orthologues. In addition, we...
IL-6 increases airway resistance in the rat
20 Jul 2010 at 1:06pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 18 July 2010
Alessandro, Rubini
The end-inspiratory occlusion method was applied in anesthetized, paralyzed, positive pressure-ventilated rats to assess the possible effects of interleukin IL-6 on respiratory mechanics in normal rats. Measurements were made in control rats and in experimental animals before and after IL-6 intraperitoneal administration (15ng/100g), including static respiratory system elastance, the resistance to airflow and to the movement of respiratory system tissues, and the resistance due to lung stress?relaxation and mechanical inhomogeneity. Respiratory system hysteresis was also measured, and total mechanical breathing work rate and its elastic and resistive components calculated.Control rats did not exhibit alteration in respiratory mechanics during the observation...
A state of acquired IL-10 deficiency in 0.4% of Danish blood donors
19 Jul 2010 at 1:09pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 17 July 2010
Carina, de Lemos Rieper , Pia, Galle , Bente Klarlund, Pedersen , Morten Bagge, Hansen
Autoantibodies against a variety of growth factors and cytokines are present in preparations of pooled normal human IgG, such as IVIg. The present study demonstrated that healthy Danish blood donors produced high concentrations of anti-IL-10 IgG antibodies that bound IL-10 with extremely high avidity. The antibodies were of IgG class, polyclonally derived and acted as competitive IL-10 inhibitors in vitro, substantially inhibiting cellular IL-10 receptor binding and neutralizing IL-10 activity in vitro. The antibodies failed to bind viral forms of IL-10 or other members of the human IL-10 family (IL-19, IL-20, IL-22, IL-24, IL-26, IL-28A, IL-28B, IL-29). The production of...
Impaired hepatic removal of interleukin-6 in patients with liver cirrhosis
15 Jul 2010 at 1:07pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 15 July 2010
Reiner, Wiest , Johanna, Weigert , Josef, Wanninger , Markus, Neumeier , Sabrina, Bauer , ...
Systemic concentrations of interleukin-6 (IL-6) are elevated in patients with liver cirrhosis, and impaired hepatic uptake of IL-6 was suggested to contribute to higher levels in these patients. To test this hypothesis IL-6 was measured in portal venous serum (PVS), hepatic venous serum (HVS) and systemic venous serum (SVS) of 41 patients with liver cirrhosis and four patients with normal liver function. IL-6 was higher in PVS than HVS of all blood donors and about 43% of portal vein derived IL-6 was extracted by the healthy liver, and 6.3% by the cirrhotic liver demonstrating markedly impaired removal of IL-6 by...
High interleukin-12 production from stimulated peripheral blood mononuclear c...
14 Jul 2010 at 1:07pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 14 July 2010
Huang-Pin, Wu , Sheng-Fong, Kuo , Shao-Yun, Wu , Duen-Yau, Chuang
Sugar control is important in patients with sepsis. Interleukin (IL)-12 induces the polarization of CD4+ T cells to the T helper 1 (Th1) phenotype. Regulatory T (Treg) cells are important in immunity and disease. The aim of this work is to determine whether hyperglycemia or insulin alters IL-12 response in peripheral mononuclear cells (PBMCs). Methods: The PBMCs from 15 type 2 diabetes mellitus (DM) patients and 13 healthy controls were used for cell analysis and culture with or without treatment by glucose and insulin or stimulation by lipopolysaccharide (LPS) for 1, 2, and 3days. Results: The IL-12 level in the supernatant of LPS-stimulated PBMCs...
Granulocyte colony stimulating factor in myocardial infarction with low eject...
6 Jul 2010 at 1:03pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 6 July 2010
Franca S., Angeli , Charles, Smith , Nicolas, Amabile , Mia, Shapiro , Lauren, Bartlett , ...
Background: We investigated the safety and efficacy of GCSF therapy in a porcine model of ischemia?reperfusion with left ventricle ejection fraction of
CXCL12 gene expression is upregulated by hypoxia and growth arrest but not by...
6 Jul 2010 at 1:03pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 6 July 2010
Begoña, Santiago , Esther, Calonge , Manuel J. Del, Rey , Irene, Gutierrez-Cañas , Elena, Izquierdo , ...
 Objectives: CXCL12 is a constitutively expressed chemokine with important homeostatic functions. Increased CXCL12 expression has been observed in several inflammatory conditions, including rheumatoid arthritis (RA). This study was undertaken to identify potential mechanisms of regulation of CXCL12 gene expression by human fibroblasts under normal or inflammatory conditions. Methods: Synovial fibroblasts (SF) were cultured from RA and osteoarthritis (OA) synovial tissues. CXCL12 mRNA expression was analysed by real time quantitative RT-PCR in RA-SF under different growth conditions, and exposed to hypoxia or to different pro-inflammatory factors. A 5?CXCL12 ?1.4kb promoter region fragment was cloned in a luciferase reporter plasmid and its activity analysed in...
Lack of utility of plasma TNF-? level in predicting therapeutic efficacy in p...
6 Jul 2010 at 1:03pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 5 July 2010
Sachin, Kumar , Randeep, Guleria , Vikas, Singh , Anant, Mohan , Alok C., Bharti , ...
 Background: Accumulating evidence suggests a change in cytokine profile after cytotoxic therapies. We hypothesized that change in plasma levels of tumor necrosis factor-alpha (TNF-?) during the course of chemotherapy in lung cancer may predict therapeutic efficacy at an early stage. Methods: Plasma TNF-? levels were quantified before first, second, and third cycle of chemotherapy in 42 patients with advanced non-small cell lung cancer and correlated with response to therapy as assessed by computed tomography after the third chemotherapy cycle. Results: Plasma levels of TNF-? measured before various treatment cycles could not differentiate among patients with remission, no change, and progression. For predicting inadequate therapeutic response,...
Elevated circulating levels of macrophage migration inhibitory factor in poly...
5 Jul 2010 at 1:07pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 3 July 2010
Frank, González , Neal S., Rote , Judi, Minium , Amy L., Weaver , John P., Kirwan
Women with polycystic ovary syndrome (PCOS) have chronic low level inflammation which can increase the risk of atherogenesis. We evaluated the status of circulating macrophage migration inhibitory factor (MIF), a proinflammatory cytokine involved in atherogenesis, in women with PCOS and weight-matched controls. Two-way analysis of variance models adjusted for age were fit to evaluate the effect of PCOS status (PCOS vs. controls) and weight-class (obese vs. lean) on MIF and other parameters. MIF levels were significantly (p
Th1 to Th2 immune deviation facilitates, but does not cause, islet allograft ...
3 Jul 2010 at 1:01pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 2 July 2010
Dong, Zhang , Alberto, Sanchez-Fueyo , Kensaku, Kawamoto , Sophoclis P., Alexopoulos , Wensheng, Zhang , ...
It has been reported that Th1 to Th2 immune deviation effectively promotes peripheral tolerance in situations involving a limited T cell clone size, such as T cell-dependent autoimmunity and transplantation across minor, but not major, histocompatibility barriers. In this study, we tested the hypothesis that while Th1 to Th2 immune deviation fails to induce tolerance in the MHC-mismatched islet allograft model, it may promote a state that is permissive for tolerance induction. Here, we report that anti-IL-12 did not prevent acute rejection of islet allografts when administered alone. In conjunction with CTLA4/Fc, however, anti-IL-12 greatly facilitated long-term engraftment in three...
Cerebrospinal fluid u-plasminogen activator and matrix metalloproteinase-9 le...
2 Jul 2010 at 1:01pm
Publication year: 2010
Source: Cytokine, In Press, Corrected Proof, Available online 2 July 2010
Oranuch, Sanpool , Pewpan M., Intapan , Tongjit, Thanchomnang , Taweesak, Santivarangkana , Kanigar, Niwattayakul , ...
Cerebrospinal fluid (CSF) urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP-9) levels were measured in patients with eosinophilic meningitis associated with angiostrongyliasis (EOMA) by quantitative sandwich enzyme immunoassays. The CSF concentrations of uPA and MMP-9 were evaluated in 30 EOMA patients and 10 controls. The CSF uPA and MMP-9 levels of the EOMA patients were significantly higher than those of the controls (p

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